LSD1 Kit ELISA

N° du produit ABIN6730575

Détail du produit LSD1 Kit ELISA

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Épitope: pSer112

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human and Mouse Phospho-LSD1 (S112) and Total LSD1 ELISA Kit. This assay semi-quantitatively measures phosphorylated LSD1 (Ser112) and Total LSD1 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human and mouse LSD1 phosphorylated at Serine-112 and total LSD1.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Autre désignation: LSD1 (KDM1A Produits)

Synonymes: AOF2 Kit ELISA, BHC110 Kit ELISA, KDM1 Kit ELISA, LSD1 Kit ELISA, 1810043O07Rik Kit ELISA, AA408884 Kit ELISA, Aof2 Kit ELISA, D4Ertd478e Kit ELISA, Kdm1 Kit ELISA, Lsd1 Kit ELISA, mKIAA0601 Kit ELISA, DKFZp459N2450 Kit ELISA, RGD1562975 Kit ELISA, aof2 Kit ELISA, kdm1 Kit ELISA, wu:fb82e06 Kit ELISA, wu:fc51a02 Kit ELISA, wu:fj36a05 Kit ELISA, wu:fj66b06 Kit ELISA, KDM1A Kit ELISA, lsd1 Kit ELISA, xLSD1 Kit ELISA, AtLSD1 Kit ELISA, F9F13.30 Kit ELISA, F9F13_30 Kit ELISA, LESION SIMULATING DISEASE Kit ELISA, LESION SIMULATING DISEASE 1 Kit ELISA, lysine demethylase 1A Kit ELISA, lysine (K)-specific demethylase 1A Kit ELISA, lysine (K)-specific demethylase 1a Kit ELISA, LSD1 zinc finger family protein Kit ELISA, KDM1A Kit ELISA, Kdm1a Kit ELISA, kdm1a Kit ELISA, LSD1 Kit ELISA

ID gène: 23028

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, L'effet Warburg