FZD2 Kit ELISA

N° du produit ABIN6730675

Détail du produit FZD2 Kit ELISA

Antigène: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Frizzled-2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Frizzled-2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FZD2 (Frizzled Family Receptor 2 (FZD2))

Autre désignation: Frizzled-2 (FZD2 Produits)

Synonymes: BG01711 Kit ELISA, CG9739 Kit ELISA, D-Fz2 Kit ELISA, D-fz2 Kit ELISA, DFZ2 Kit ELISA, DFz-2 Kit ELISA, DFz2 Kit ELISA, Dfrz2 Kit ELISA, Dfz2 Kit ELISA, Dfz[[2]] Kit ELISA, Dm Fz2 Kit ELISA, Dmel\\CG9739 Kit ELISA, FZ2 Kit ELISA, Fz2 Kit ELISA, dFz2 Kit ELISA, dfz(2) Kit ELISA, dfz2 Kit ELISA, dfz2r Kit ELISA, frz2 Kit ELISA, fz-2 Kit ELISA, fz8 Kit ELISA, zfz2 Kit ELISA, zg08 Kit ELISA, cb383 Kit ELISA, wu:fb70g09 Kit ELISA, FZD2 Kit ELISA, fzE2 Kit ELISA, hFz2 Kit ELISA, Fz-10 Kit ELISA, Fzd10 Kit ELISA, cFz-2 Kit ELISA, frizzled2 Kit ELISA, fz2 Kit ELISA, xfz2 Kit ELISA, AL033370 Kit ELISA, AW456835 Kit ELISA, Fz10 Kit ELISA, Mfz10 Kit ELISA, Mfz10a Kit ELISA, frizzled 2 Kit ELISA, frizzled class receptor 2 Kit ELISA, frizzled class receptor 2 S homeolog Kit ELISA, fz2 Kit ELISA, fzd2 Kit ELISA, FZD2 Kit ELISA, Fzd2 Kit ELISA, fzd2.S Kit ELISA

UniProt: Q9JIP6

Pathways: Signalisation WNT