EPH Receptor A4 Kit ELISA

N° du produit ABIN6730704

Détail du produit EPH Receptor A4 Kit ELISA

Antigène: EPH Receptor A4 (EPHA4)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse EphA4 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse EphA4.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EPH Receptor A4 (EPHA4)

Autre désignation: EphA4 (EPHA4 Produits)

Synonymes: HEK8 Kit ELISA, SEK Kit ELISA, TYRO1 Kit ELISA, RGD1560587 Kit ELISA, ephe Kit ELISA, EPHA4 Kit ELISA, Ci-Eph1 Kit ELISA, sek Kit ELISA, hek8 Kit ELISA, rtk1 Kit ELISA, tyr01 Kit ELISA, 2900005C20Rik Kit ELISA, AI385584 Kit ELISA, Cek8 Kit ELISA, Hek8 Kit ELISA, Sek Kit ELISA, Sek1 Kit ELISA, Tyro1 Kit ELISA, rb Kit ELISA, XEpha4 Kit ELISA, sek-1 Kit ELISA, epha4 Kit ELISA, pag Kit ELISA, EphA4 Kit ELISA, apha3 Kit ELISA, ek2 Kit ELISA, epha3 Kit ELISA, sb:cb246 Kit ELISA, zek2 Kit ELISA, zgc:109882 Kit ELISA, EPH receptor A4 Kit ELISA, Eph receptor A4 Kit ELISA, ephrin receptor epsilon Kit ELISA, EPH receptor A4 S homeolog Kit ELISA, EPH receptor A4 L homeolog Kit ELISA, eph receptor A4a Kit ELISA, EPHA4 Kit ELISA, Epha4 Kit ELISA, ephe Kit ELISA, epha4 Kit ELISA, epha4.S Kit ELISA, epha4.L Kit ELISA, epha4a Kit ELISA

UniProt: Q03137

Pathways: Signalisation RTK