P4HB Kit ELISA

N° du produit ABIN6730762

Détail du produit P4HB Kit ELISA

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human P4HB ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human P4HB.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: P4HB (P4HB Produits)

Synonymes: DSI Kit ELISA, ERBA2L Kit ELISA, GIT Kit ELISA, P4Hbeta Kit ELISA, PDI Kit ELISA, PDIA1 Kit ELISA, PHDB Kit ELISA, PO4DB Kit ELISA, PO4HB Kit ELISA, PROHB Kit ELISA, PDA2 Kit ELISA, PDIP Kit ELISA, PDIR Kit ELISA, ERp59 Kit ELISA, Pdia1 Kit ELISA, Thbp Kit ELISA, p55 Kit ELISA, XPDIp Kit ELISA, pdi Kit ELISA, 1810041F13Rik Kit ELISA, AI661267 Kit ELISA, Pdip Kit ELISA, Pdipl Kit ELISA, p58 Kit ELISA, prolyl 4-hydroxylase subunit beta Kit ELISA, protein disulfide isomerase family A member 2 Kit ELISA, prolyl 4-hydroxylase, beta polypeptide Kit ELISA, protein disulfide isomerase family A member 2 L homeolog Kit ELISA, protein disulfide isomerase associated 2 Kit ELISA, prolyl 4-hydroxylase subunit beta L homeolog Kit ELISA, protein disulfide-isomerase Kit ELISA, P4HB Kit ELISA, PDIA2 Kit ELISA, P4hb Kit ELISA, pdia2.L Kit ELISA, Pdia2 Kit ELISA, p4hb.L Kit ELISA, LOC8281065 Kit ELISA

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism