Sonic Hedgehog Kit IQ-ELISA

N° du produit ABIN6730851

Détail du produit Sonic Hedgehog Kit IQ-ELISA

Antigène: Sonic Hedgehog (SHH)

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) Sonic Hedgehog N-Terminal ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: 4.2 pg/ml in Assay Diluent C, and8 pg/ml in Assay Diluent B. (Anticipated minimum sensitivity. Assay may detect lower levels of antigen.)

Sensibilité: 4.2 pg/mL

Attributs du produit: The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human Sonic Hedgehog N-Terminal IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Sonic Hedgehog N-Terminal in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Sonic Hedgehog N-Terminal coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Sonic Hedgehog N-Terminal present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

Ingrédients:

• Sonic Hedgehog N-Terminal Microplate (Item A): 96 well PCR plate coated with anti-Human Sonic Hedgehog N-Terminal
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Sonic Hedgehog N-Terminal
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Sonic Hedgehog N-Terminal (Item F): 2 vials of a 4x concentrated solution of anti-Human Sonic Hedgehog N-Terminal affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

Features:
- Excellent for follow up/confirmation of antibody array data
- Pre-coated 96 well plate
- Strip plate format minimizes waste
- Complete kit includes all necessary reagents
- 10x more sensitive than ELISA
- Reproducible and reliable
- Optimized for serum, plasma, urine and cell culture medium
- 1/10th the sample volume of an ELISA

Research Applications:
- Detection of quantitative protein levels in biological fluids
- Validation of antibody array results
- Validation of biomarker discovery studies

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2°C to 8°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: Sonic Hedgehog (SHH Produits)

Synonymes: HHG1 Kit IQ-ELISA, HLP3 Kit IQ-ELISA, HPE3 Kit IQ-ELISA, MCOPCB5 Kit IQ-ELISA, SMMCI Kit IQ-ELISA, TPT Kit IQ-ELISA, TPTPS Kit IQ-ELISA, 9530036O11Rik Kit IQ-ELISA, Dsh Kit IQ-ELISA, Hhg1 Kit IQ-ELISA, Hx Kit IQ-ELISA, Hxl3 Kit IQ-ELISA, M100081 Kit IQ-ELISA, fc83d08 Kit IQ-ELISA, shh Kit IQ-ELISA, syu Kit IQ-ELISA, vhh-1 Kit IQ-ELISA, vhh1 Kit IQ-ELISA, wu:fc83d08 Kit IQ-ELISA, Xhh Kit IQ-ELISA, hedgehog Kit IQ-ELISA, xshh Kit IQ-ELISA, SHH Kit IQ-ELISA, twh Kit IQ-ELISA, twhh Kit IQ-ELISA, sonic hedgehog Kit IQ-ELISA, sonic hedgehog a Kit IQ-ELISA, sonic hedgehog L homeolog Kit IQ-ELISA, sonic hedgehog protein A Kit IQ-ELISA, sonic hedgehog b Kit IQ-ELISA, SHH Kit IQ-ELISA, Shh Kit IQ-ELISA, shha Kit IQ-ELISA, shh.L Kit IQ-ELISA, shh Kit IQ-ELISA, shhb Kit IQ-ELISA

ID gène: 6469

UniProt: Q15465

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development