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FRS2 Kit ELISA

FRS2 Reactivité: Humain, Souris pTyr436 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
N° du produit ABIN6810516
  • Antigène Voir toutes FRS2 Kits ELISA
    FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
    Épitope
    pTyr436
    Reactivité
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human and Mouse Phospho-FRS2 (Tyr436) ELISA Kit. This assay semi-quantitatively measures FRS2 phosphorylated at Tyrosine-436 in cell lysate samples.
    Type d'échantillon
    Cell Samples, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human and Mouse FRS2 phosphorylated at site Tyrosine-436.
    Attributs du produit
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes FRS2 Kits ELISA
    FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
    Autre désignation
    FRS2 (FRS2 Produits)
    Synonymes
    frs2 Kit ELISA, wu:fd21h10 Kit ELISA, zgc:91973 Kit ELISA, FRS2A Kit ELISA, FRS2alpha Kit ELISA, SNT Kit ELISA, SNT-1 Kit ELISA, SNT1 Kit ELISA, 4732458E18 Kit ELISA, C330018A15Rik Kit ELISA, Frs2alpha Kit ELISA, fibroblast growth factor receptor substrate 2 Kit ELISA, fibroblast growth factor receptor substrate 2b Kit ELISA, FRS2 Kit ELISA, frs2b Kit ELISA, Frs2 Kit ELISA
    ID gène
    10818
    UniProt
    Q8WU20, Q8C180
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Signaling Events mediated by VEGFR1 and VEGFR2
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