H2AFX Kit ELISA

N° du produit ABIN6810525

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Souris et Rat H2AFX.

Aperçu rapide pour H2AFX Kit ELISA (ABIN6810525)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Épitope: acetylated

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Lysate

Détail du produit H2AFX Kit ELISA

Fonction: Human, Mouse and Rat Acetylated-Histone H2AX ELISA Kit. This ELISA is for measuring Acetylated-Histone H2AX in human, mouse and rat cell lysates.

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human

Attributs du produit:

• Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Assay Diluent
• Anti-Acetylated Antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Assay Diluent
• Anti-Acetylated Antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 ^°C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at â€""20^°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4^°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at â€""20^°C. Item D, store at 2-8^°C for up to one month (store at -20^°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70^°C.

Date de péremption: 6 months

Détail du antigène

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2AX

Sujet:

Gene Symbols: H2AFX, H2AX, H2A.X, HIST5-2AX

Background: Acetylated-Histone H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining Acetylated Histone H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat Acetylated-Histone H2AX. An anti-pan Histone H2AX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Histone H2AX present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti- Acetyl Lysine antibody is used to detect Acetylated Histone H2AX. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Acetylated Histone H2AX bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): H2a/x H2A.X

ID gène: 3014

UniProt: P16104, P27661

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus