GCAP1 Kit ELISA

N° du produit ABIN6951132

Détail du produit GCAP1 Kit ELISA

Antigène: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GUCA1A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: GCAP1 (GUCA1A) (Guanylate Cyclase Activator 1A (Retina) (GUCA1A))

Autre désignation: GUCA1A (GUCA1A Produits)

Synonymes: C6orf131 Kit ELISA, COD3 Kit ELISA, CORD14 Kit ELISA, GCAP Kit ELISA, GCAP1 Kit ELISA, GUCA Kit ELISA, GUCA1 Kit ELISA, dJ139D8.6 Kit ELISA, MGC84170 Kit ELISA, GUCA1A Kit ELISA, guca1a Kit ELISA, MGC146317 Kit ELISA, GC-A Kit ELISA, Gcap1 Kit ELISA, Guca1 Kit ELISA, mGCAP1 Kit ELISA, gcap1 Kit ELISA, guanylate cyclase activator 1A Kit ELISA, guanylate cyclase activator 1A L homeolog Kit ELISA, guanylate cyclase activator 1A (retina) Kit ELISA, guanylate cyclase activator 1a (retina) Kit ELISA, GUCA1A Kit ELISA, Guca1a Kit ELISA, guca1a.L Kit ELISA, guca1a Kit ELISA

ID gène: 2978

UniProt: P43080

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction