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MPL Kit ELISA

MPL Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951177
  • Antigène Voir toutes MPL Kits ELISA
    MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Thrombopoietin r/TPO-R ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product MPL Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes MPL Kits ELISA
    MPL (Myeloproliferative Leukemia Virus Oncogene (MPL))
    Autre désignation
    Thrombopoietin r (TPO-R (MPL Produits)
    Synonymes
    C-MPL Kit ELISA, CD110 Kit ELISA, MPLV Kit ELISA, THCYT2 Kit ELISA, TPOR Kit ELISA, TPO-R Kit ELISA, c-mpl Kit ELISA, c-mpl-I Kit ELISA, c-mpl-II Kit ELISA, hlb219 Kit ELISA, MPL Kit ELISA, mpll Kit ELISA, tpor Kit ELISA, tpor.b Kit ELISA, MPL proto-oncogene, thrombopoietin receptor Kit ELISA, myeloproliferative leukemia virus oncogene Kit ELISA, MPL Kit ELISA, Mpl Kit ELISA, mpl Kit ELISA
    ID gène
    4352
    UniProt
    P40238
    Pathways
    Signalistation JAK/STAT
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