SOCS6 Kit ELISA

N° du produit ABIN6951237

Détail du produit SOCS6 Kit ELISA

Antigène: SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human socs6 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: SOCS6 (Suppressor of Cytokine Signaling 6 (SOCS6))

Autre désignation: socs6 (SOCS6 Produits)

Synonymes: CIS-4 Kit ELISA, CIS4 Kit ELISA, HSPC060 Kit ELISA, SOCS-4 Kit ELISA, SOCS-6 Kit ELISA, SOCS4 Kit ELISA, SSI4 Kit ELISA, STAI4 Kit ELISA, STATI4 Kit ELISA, socs6 Kit ELISA, MGC81477 Kit ELISA, SOCS6 Kit ELISA, 1500012M23Rik Kit ELISA, 5830401B18Rik Kit ELISA, AI447482 Kit ELISA, Cis4 Kit ELISA, Cish4 Kit ELISA, Socs4 Kit ELISA, socs6l Kit ELISA, zgc:64107 Kit ELISA, wu:fb77f06 Kit ELISA, suppressor of cytokine signaling 6 Kit ELISA, suppressor of cytokine signaling 6 L homeolog Kit ELISA, suppressor of cytokine signaling Kit ELISA, suppressor of cytokine signaling 6b Kit ELISA, SOCS6 Kit ELISA, Socs6 Kit ELISA, socs6.L Kit ELISA, socs6 Kit ELISA, socs6b Kit ELISA

ID gène: 9306

UniProt: O14544

Pathways: Signalistation JAK/STAT, Carbohydrate Homeostasis