TWIST1 Kit ELISA

N° du produit ABIN6951241

Détail du produit TWIST1 Kit ELISA

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human TWIST1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Autre désignation: TWIST1 (TWIST1 Produits)

Synonymes: scs Kit ELISA, Xtwi Kit ELISA, acs3 Kit ELISA, X-twi Kit ELISA, bpes2 Kit ELISA, bpes3 Kit ELISA, twist Kit ELISA, Xtwist Kit ELISA, MGC69439 Kit ELISA, twist1 Kit ELISA, ACS3 Kit ELISA, BPES2 Kit ELISA, BPES3 Kit ELISA, CRS1 Kit ELISA, SCS Kit ELISA, TWIST Kit ELISA, bHLHa38 Kit ELISA, AA960487 Kit ELISA, M-Twist Kit ELISA, Pde Kit ELISA, Ska10 Kit ELISA, Ska Kit ELISA, Twist Kit ELISA, pdt Kit ELISA, cTwist Kit ELISA, Twist1b Kit ELISA, twist-1 Kit ELISA, wu:fd04c01 Kit ELISA, zgc:101115 Kit ELISA, twist family bHLH transcription factor 1 Kit ELISA, twist family bHLH transcription factor 1 S homeolog Kit ELISA, twist basic helix-loop-helix transcription factor 1 Kit ELISA, twist homolog 1 (Drosophila) Kit ELISA, twist family bHLH transcription factor 1a Kit ELISA, TWIST1 Kit ELISA, twist1 Kit ELISA, twist1.S Kit ELISA, Twist1 Kit ELISA, twist1a Kit ELISA

ID gène: 7291

UniProt: Q15672

Pathways: Signalisation p53, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity