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CDK2AP2 Kit ELISA

CDK2AP2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951488
  • Antigène Voir toutes CDK2AP2 Kits ELISA
    CDK2AP2 (Cyclin-Dependent Kinase 2 Associated Protein 2 (CDK2AP2))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human CDK2AP2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes CDK2AP2 Kits ELISA
    CDK2AP2 (Cyclin-Dependent Kinase 2 Associated Protein 2 (CDK2AP2))
    Autre désignation
    CDK2AP2 (CDK2AP2 Produits)
    Synonymes
    DOC-1R Kit ELISA, p14 Kit ELISA, zgc:92182 Kit ELISA, 5830466O21Rik Kit ELISA, D19Ertd144e Kit ELISA, Doc-1r Kit ELISA, cyclin dependent kinase 2 associated protein 2 Kit ELISA, cyclin-dependent kinase 2 associated protein 2 Kit ELISA, CDK2-associated protein 2 Kit ELISA, CDK2AP2 Kit ELISA, cdk2ap2 Kit ELISA, Cdk2ap2 Kit ELISA
    ID gène
    10263
    UniProt
    O75956
    Pathways
    Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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