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C4BPA Kit ELISA (Complement Component 4 Binding Protein, alpha) Kit ELISA

C4BPA Reactivité: Humain Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Pubmed (1)
N° du produit ABIN6973975
$788.33
Plus shipping costs $45.00
96 tests
local_shipping Destination: Etats-Unis
Available
  • Antigène
    C4BPA
    Reactivité
    Humain
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Fonction
    For the quantitative determination of human C4 binding protein (C4BP) concentrations in serum, plasma, tissue homogenates.
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of human C4BP. No significant cross-reactivity or interference between human C4BP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human C4BP and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    0.156 ng/mL
    Ingrédients
    • Assay plate
    • Standard
    • HRP-avidin (100 x concentrate)
    • Biotin-antibody (100 x concentrate)
    • Sample Diluent
    • HRP-avidin Diluent
    • Biotin-antibody Diluent
    • Wash Buffer (25 x concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Préparation des réactifs
    1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
    2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
    3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
    4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 40 ng/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (40 ng/mL). Sample Diluent serves as the zero standard (0 ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25 °C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
    • Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.
    Précision du teste
    Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.
    Date de péremption
    6 months
  • Hedegaard, Sellebjerg, Krakauer, Hesse, Bendtzen, Nielsen: "Interferon-beta increases systemic BAFF levels in multiple sclerosis without increasing autoantibody production." dans: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 17, Issue 5, pp. 567-77, 2011 (PubMed).

  • Antigène
    C4BPA
    Autre désignation
    complement component 4 binding protein, alpha (C4BPA ELISA Kit Extrait)
    Synonymes
    CRES, c4bp, C4BP, PRP, complement component 4 binding protein, alpha chain, C4b-binding protein alpha chain, complement component 4 binding protein alpha, complement component 4 binding protein, alpha, LOC419851, c4bp, C4BPA, C4bpa
    Sujet

    Abbreviation: C4BPA

    Alias: RP11-164O23.4, C4BP, PRP, C4b binding protein, alpha chain|complement component 4 binding protein, alpha chain|complement component 4-binding protein, alpha|proline-rich protein

    UniProt
    P04003
    Pathways
    Système du Complément
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