SARS-CoV-2 N-Protein IgM Antibody Kit ELISA

N° du produit ABIN7666570

Ce kit ELISA Humain est un kit ELISA Colorimetric conçu pour quantifier Humain .

Aperçu rapide pour SARS-CoV-2 N-Protein IgM Antibody Kit ELISA (ABIN7666570)

Antigène: SARS-CoV-2 N-Protein IgM Antibody

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit

Fonction: anti-SARS-CoV-2 (NP) IgM - ELISA kit for the detection of IgM antibodies to nucleocapsid protein (NP) of coronavirus SARS-CoV-2, causing the COVID-19 disease in human serum and plasma isolated from venous or capillary blood.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control serum, r.t.u. NC 1 vial
• 1.3 mL Positive control serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator, r.t.u. 1) CAL 1 vial
• 13 mL Anti-human IgM antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
• 2 mL RF sorbent 2), 25x concentrated RF SORB 25x 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
• 13 mL Stop solution, r.t.u. STOP 1 vial
• Instruction manual
• Certificate
• of quality 1) r.t.u., ready to use 2) Goat anti-human IgG globulin

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-SARS-CoV-2 (NP) IgM is a solid-phase immunoanalytical test. The specific antigen (Nucleocapsid Protein) is fixed to each well of the microtiter strips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgM conjugate during the second incubation. The unbound peroxidase conjugate is then removed and TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: SARS-CoV-2 N-Protein IgM Antibody

Autre désignation: SARS-CoV-2 NP IgM

Classe de substances: Antibody