PTH Kit ELISA

N° du produit ABIN7672009

Le kit ELISA PTH (ABIN7672009) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PTH.

Aperçu rapide pour PTH Kit ELISA (ABIN7672009)

Antigène: PTH (Parathyroid Hormone (PTH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Classe de qualité: Quick

Détail du produit PTH Kit ELISA

Seuil minimal de détection: 15.625 pg/mL

Fonction: Human PTH 1-34/Teriparatide (Parathyroid hormone 1-34) QuickTest ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with PTH 1-34/Teriparatide , no obvious cross reaction with other analogues.

Sensibilité: 9.375 pg/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Cap/Det Ab (Ready to use
• Red)
• TMB Substrate
• Sample Dilution Buffer (blue)
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• Microorifice oscillator
• or shake bed 37 °C incubator (When using a water bath incubator
• ensure the internal air temperature is 35-38 °C, When using the cell CO2 incubator
• use a sealed bag to isolate the microplate)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antibody

Durée du test: 2 h

Plaque: Pre-coated

Procédure de l'essai:

1. Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
2. Wash the plate twice without immersion.
3. Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
4. Wash the plate five times without immersion.
5. Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
6. Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: PTH (Parathyroid Hormone (PTH))

Autre désignation: PTH 1-34

Classe de substances: Hormone

Sujet: Parathyroid hormone, PTH, Parathormone, Parathyrin, PTH

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process