Leptin Receptor Kit ELISA

N° du produit ABIN7826739

Kit ELISA Leptin Receptor Souris, Colorimetric test pour la quantification de Souris Leptin Receptor.

Aperçu rapide pour Leptin Receptor Kit ELISA (ABIN7826739)

Antigène: Leptin Receptor (LEPR)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.13 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Leptin Receptor Kit ELISA

Seuil minimal de détection: 3.13 ng/mL

Fonction: This ELISA kit applies to the in vitro quantitative determination of Mouse LEPR concentrations in serum, plasma.

Analytical Method: Quantitative

Réactivité croisée (Details): This kit recognizes Mouse LEPR in samples.No significant cross-reactivity or interference between Mouse LEPR and analogues was observed

Attributs du produit: Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

Ingrédients:

• Capture Antibody
• Reference Standard
• Micro ELISA pre-Plate
• Biotinylated Detection Antibody
• HRP Conjugate
• Manual

Matériel non inclus:

• Ancillary Reagents (ABIN7670776)
• Microplate reader with 450 nm wavelength filter
• High-precision transfer pipette
• EP tubes
• disposable pipette tips
• Incubator capable of maintaining 37 °C
• Deionized or distilled water
• Absorbent paper
• Loading slot

Information d'application

Volume d'échantillon: 100 µL

Durée du test: 1 d

Plaque: Uncoated

Protocole:

1. Take out the Micro ELISA pre-Plate, add 100 µL Capture antibody working solution to each well. Incubate overnight at 2-8°C.
   Discard the liquid, add 200 µL ELISA Plate Blocking Buffer to each well. Incubate for 60 min at 37°C
2. Discard the liquid, add 100 µL standard or sample to the wells. Incubate for 90 min at 37°C
3. Discard the liquid, immediately add 100 µL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
4. Aspirate and wash the plate 3 times
5. Add 100 µL HRP conjugate working solution. Incubate foe 30 min at 37°C. Aspirate and wash the plate for 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37°C
7. Add 50 µL Stop Solution
8. Read the plate at 450 nm immediately. Calculation of the results

Précision du teste: Both intra-CV and inter-CV are < 10%

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Store all unopend components at -20°C for up to 12 months.

Date de péremption: 12 months

Détail du antigène

Antigène: Leptin Receptor (LEPR)

Autre désignation: LEPR

Sujet: CD295,LEP-R,LR,LEPRD,OB-R,OBR,

UniProt: P48356

Pathways: Signalistation JAK/STAT, AMPK Signaling, Feeding Behaviour