14-3-3 zeta Kit ELISA
Aperçu rapide pour 14-3-3 zeta Kit ELISA (ABIN7830382)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- YWHAZ/YWHAB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of YWHAZ/YWHAB expression in cultured cells.
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Analytical Method
- Qualitative
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Réactivité croisée
- Souris, Rat
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- 14-3-3 zeta (YWHAZ)
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Autre désignation
- YWHAZ
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Sujet
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Synonyms: KCIP-1|MGC111427|MGC126532|MGC138156
Gene Description: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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ID gène
- 7534
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UniProt
- P63104
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Pathways
- Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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