beta 2 Adrenergic Receptor Kit ELISA
Aperçu rapide pour beta 2 Adrenergic Receptor Kit ELISA (ABIN7830402)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- ADRB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ADRB2 expression in cultured cells.
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Analytical Method
- Qualitative
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Uncoated
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Protocole
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- Store the kit at 4°C.
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- beta 2 Adrenergic Receptor (ADRB2) (Adrenergic, beta-2-, Receptor, Surface (ADRB2))
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Autre désignation
- ADRB2
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Sujet
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Synonyms: ADRB2R|ADRBR|B2AR|BAR|BETA2AR
Gene Description: adrenergic, beta-2-, receptor, surface
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ID gène
- 154
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UniProt
- P07550
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Pathways
- cAMP Metabolic Process, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Brown Fat Cell Differentiation
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