PRKAB1 Kit ELISA

N° du produit ABIN7830411

Le kit ELISA PRKAB1 (ABIN7830411) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PRKAB1.

Aperçu rapide pour PRKAB1 Kit ELISA (ABIN7830411)

Antigène: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit PRKAB1 Kit ELISA

Fonction: PRKAB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKAB1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Autre désignation: PRKAB1

Sujet:

Synonyms: AMPK|HAMPKb|MGC17785

Gene Description: protein kinase, AMP-activated, beta 1 non-catalytic subunit

ID gène: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, L'effet Warburg