AFT1 Kit ELISA

N° du produit ABIN7830425

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain AFT1.

Aperçu rapide pour AFT1 Kit ELISA (ABIN7830425)

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit AFT1 Kit ELISA

Fonction: ATF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ATF1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Autre désignation: ATF1

Sujet:

Synonyms: EWS-ATF1|FUS/ATF-1|TREB36

Gene Description: activating transcription factor 1

ID gène: 466

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades