BID Kit ELISA

N° du produit ABIN7830443

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain BID.

Aperçu rapide pour BID Kit ELISA (ABIN7830443)

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit BID Kit ELISA

Fonction: BID (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of BID expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Autre désignation: BID

Sujet:

Synonyms: FP497|MGC15319|MGC42355

Gene Description: BH3 interacting domain death agonist

ID gène: 637

UniProt: P55957

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity