Cyclin E1 Kit ELISA

N° du produit ABIN7830542

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Cyclin E1.

Aperçu rapide pour Cyclin E1 Kit ELISA (ABIN7830542)

Antigène: Cyclin E1 (CCNE1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Cyclin E1 Kit ELISA

Fonction: CCNE1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNE1 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Cyclin E1 (CCNE1)

Autre désignation: CCNE1

Sujet:

Synonyms: CCNE

Gene Description: cyclin E1

ID gène: 898

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases