ICAM1 Kit ELISA

N° du produit ABIN7830593

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain ICAM1.

Aperçu rapide pour ICAM1 Kit ELISA (ABIN7830593)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit ICAM1 Kit ELISA

Fonction: ICAM1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ICAM1 expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM1

Sujet:

Synonyms: BB2|CD54|P3.58

Gene Description: intercellular adhesion molecule 1

ID gène: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling