Glucagon Kit ELISA

N° du produit ABIN7830676

Kit ELISA Glucagon Humain, Colorimetric test pour la quantification de Humain Glucagon.

Aperçu rapide pour Glucagon Kit ELISA (ABIN7830676)

Antigène: Glucagon (GCG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Glucagon Kit ELISA

Fonction: GCG (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GCG expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Glucagon (GCG)

Autre désignation: GCG

Sujet:

Synonyms: GLP1|GLP2|GRPP

Gene Description: glucagon

ID gène: 2641

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling