GRB10 Kit ELISA

N° du produit ABIN7830686

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain GRB10.

Aperçu rapide pour GRB10 Kit ELISA (ABIN7830686)

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit GRB10 Kit ELISA

Fonction: GRB10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRB10 expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Autre désignation: GRB10

Sujet:

Synonyms: GRB-IR|Grb-10|IRBP|KIAA0207|MEG1|RSS

Gene Description: growth factor receptor-bound protein 10

ID gène: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2