NFKBIB Kit ELISA

N° du produit ABIN7830710

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain NFKBIB.

Aperçu rapide pour NFKBIB Kit ELISA (ABIN7830710)

Antigène: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit NFKBIB Kit ELISA

Fonction: NFKBIB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NFKBIB expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Autre désignation: NFKBIB

Sujet:

Synonyms: IKBB|TRIP9

Gene Description: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta

ID gène: 4793

UniProt: Q15653

Pathways: Signalisation NF-kappaB, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades