LAT Kit ELISA

N° du produit ABIN7830734

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain LAT.

Aperçu rapide pour LAT Kit ELISA (ABIN7830734)

Antigène: LAT (Linker For Activation of T Cells (LAT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit LAT Kit ELISA

Fonction: LAT (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LAT expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: LAT (Linker For Activation of T Cells (LAT))

Autre désignation: LAT

Sujet:

Synonyms: LAT1|pp36

Gene Description: linker for activation of T cells

ID gène: 27040

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway