SKP2 Kit ELISA

N° du produit ABIN7830890

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain SKP2.

Aperçu rapide pour SKP2 Kit ELISA (ABIN7830890)

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit SKP2 Kit ELISA

Fonction: SKP2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SKP2 expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Autre désignation: SKP2

Sujet:

Synonyms: FBL1|FBXL1|FLB1|MGC1366

Gene Description: S-phase kinase-associated protein 2 (p45)

ID gène: 6502

Pathways: Mitotic G1-G1/S Phases