Topoisomerase II alpha Kit ELISA

N° du produit ABIN7830920

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Topoisomerase II alpha.

Aperçu rapide pour Topoisomerase II alpha Kit ELISA (ABIN7830920)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit Topoisomerase II alpha Kit ELISA

Fonction: TOP2A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TOP2A expression in cultured cells.

Analytical Method: Qualitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store the kit at 4°C.

Détail du antigène

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Sujet:

Synonyms: TOP2|TP2A

Gene Description: topoisomerase (DNA) II alpha 170 kDa

ID gène: 7153

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases