AKAP10 Kit ELISA

N° du produit ABIN7831039

Kit ELISA AKAP10 Humain, Colorimetric test pour la quantification de Humain AKAP10.

Aperçu rapide pour AKAP10 Kit ELISA (ABIN7831039)

Antigène: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Type d'échantillon: Cell Samples, Suspension Cell Culture

Détail du produit AKAP10 Kit ELISA

Fonction: AKAP10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKAP10 expression in cultured cells.

Analytical Method: Qualitative

Réactivité croisée: Souris, Rat

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Uncoated

Protocole:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 4°C for six months.

Détail du antigène

Antigène: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Autre désignation: AKAP10

Sujet:

Synonyms: D-AKAP2|MGC9414|PRKA10

Gene Description: A kinase (PRKA) anchor protein 10

ID gène: 11216

UniProt: O43572

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling