ICAM1 Kit ELISA

N° du produit ABIN7832841

Le kit ELISA ICAM1 (ABIN7832841) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain ICAM1.

Aperçu rapide pour ICAM1 Kit ELISA (ABIN7832841)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Biological Fluids, Plasma, Serum

Classe de qualité: QuickTest

Détail du produit ICAM1 Kit ELISA

Fonction: FastELISA ICAM-1/CD54 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human ICAM-1/CD54 (intercellular adhesion molecule 1) in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C

Stockage commentaire: Store at 2-8C for 6 months.Avoid repeated freezing and thawing.

Date de péremption: 6 months

Détail du antigène

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: ICAM-1/CD54

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling