Ephrin A1 Kit ELISA

N° du produit ABIN7832859

Le kit ELISA Ephrin A1 (ABIN7832859) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Ephrin A1.

Aperçu rapide pour Ephrin A1 Kit ELISA (ABIN7832859)

Antigène: Ephrin A1 (EFNA1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Classe de qualité: QuickTest

Détail du produit Ephrin A1 Kit ELISA

Fonction: FastELISA EFNA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human EFNA1 (Ephrin A1) in Serum, plasma.

Analytical Method: Quantitative

Specificité: This assay is highly specific for detection of Human EFNA1. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Date de péremption: 6 months

Détail du antigène

Antigène: Ephrin A1 (EFNA1)

Autre désignation: EFNA1

Sujet:

Synonyms: B61|ECKLG|EFL1|EPLG1|LERK1|TNFAIP4

Gene Description: ephrin-A1

ID gène: 1942

Pathways: Signalisation RTK