GDF15 Kit ELISA
Aperçu rapide pour GDF15 Kit ELISA (ABIN7832861)
Antigène
Voir toutes GDF15 Kits ELISAReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
Classe de qualité
-
-
Fonction
- FastELISA GDF15 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human GDF15 (Growth differentiation factor 15) in Serum, plasma.
-
Analytical Method
- Quantitative
-
Specificité
- This assay is highly specific for detection of Human GDF15. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
-
-
-
-
Indications d'application
- Optimal working dilution should be determined by the investigator.
-
Plaque
- Pre-coated
-
Protocole
-
- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
-
Restrictions
- For Research Use only
-
-
-
Conseil sur la manipulation
- Avoid repeated freezing and thawing
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
-
Date de péremption
- 6 months
-
-
- GDF15 (Growth Differentiation Factor 15 (GDF15))
-
Autre désignation
- GDF15
-
Sujet
-
Synonyms: GDF-15|MIC-1|MIC1|NAG-1|PDF|PLAB|PTGFB
Gene Description: growth differentiation factor 15
-
ID gène
- 9518
-
Pathways
- SARS-CoV-2 Protein Interactome
Antigène Voir toutes GDF15 Kits ELISA
-