SAA1 Kit ELISA
Aperçu rapide pour SAA1 Kit ELISA (ABIN7832881)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
Classe de qualité
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Fonction
- FastELISA SAA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human SAA (Serum Amyloid A) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificité
- This assay is highly specific for detection of Human SAA. No significant cross-reactivity or interference between Human SAA and analogues was observed.
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid repeated freezing and thawing
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Date de péremption
- 6 months
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- SAA1 (Serum Amyloid A1 (SAA1))
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Autre désignation
- SAA1
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Sujet
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Synonyms: MGC111216|PIG4|SAA|TP53I4
Gene Description: serum amyloid A1
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ID gène
- 6288
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Pathways
- Toll-Like Receptors Cascades
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