PLA2G2A Kit ELISA

N° du produit ABIN7833057

Kit ELISA PLA2G2A Humain, Colorimetric test pour la quantification de Humain PLA2G2A.

Aperçu rapide pour PLA2G2A Kit ELISA (ABIN7833057)

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6.25 pg/mL - 400 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit PLA2G2A Kit ELISA

Seuil minimal de détection: 6.25 pg/mL

Fonction: Sandwich ELISA for quantitative detection of Human PLA2G2A concentrations.

Analytical Method: Quantitative

Sensibilité: 3 pg/mL

Ingrédients:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Matériel non inclus:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid freeze/thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Autre désignation: PLA2G2A

Sujet: Alternative Names: PLA2B, PLA2L, RASF-A, Phospholipase A2 membrane associated, GIIC sPLA2, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2, NPS-PLA2, Phosphatidylcholine 2-acylhydrolase 2A

ID gène: 5320

UniProt: P14555

Pathways: Stem Cell Maintenance, Inositol Metabolic Process