Glucokinase (GCK) Activity Colorimetric Assay Kit

N° du produit ABIN7850002

Aperçu rapide pour Glucokinase (GCK) Activity Colorimetric Assay Kit (ABIN7850002)

Antigène: GCK (Glucokinase (Hexokinase 4) (GCK))

Méthode de détection: Colorimetric

Application: Biochemical Assay (BCA)

Type d'échantillon: Cell Samples, Tissue Samples

Analytical Method: Quantitative

Détail du produit

Gamme de detection: 2.62 U/L - 42.8 U/L

Seuil minimal de détection: 2.62 U/L

Fonction: Glucokinase (GCK) Activity Colorimetric Assay Kit

Attributs du produit: Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance in internal. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet β-cell, and has a stronger affinity for glucose than other hexokinases. Therefore, the total hexokinase activity in the sample is measured at high glucose concentration, and the hexokinase activity with low glucose affinity is measured at low glucose concentration. The GCK activity in the sample can be obtained by subtracting the total hexokinase from the total hexokinase. The detection principle of this kit: GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The activity of GCK can be calculated by measuring the generation rate of chromogenic substance at 450 nm.

Information d'application

Durée du test: 0.5 h

Précision du teste:

Inter-assay precision: 1.3-7.4%

Intra-assay precision: 2.9-6.6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Protect from light.

Stock: -20 °C

Stockage commentaire: This product can be stored at -20°C for 12 months with shading light.

Date de péremption: 12 months

Détail du antigène

Antigène: GCK (Glucokinase (Hexokinase 4) (GCK))

Autre désignation: Glucokinase