IL4 Receptor Kit ELISA

N° du produit ABIN8055978

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Cheval IL4 Receptor.

Aperçu rapide pour IL4 Receptor Kit ELISA (ABIN8055978)

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reactivité: Cheval

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit IL4 Receptor Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich Equine IL-4 ELISA Kit to quantitate Equine IL-4 in cell culture supernatants, serum, plasma(heparin, EDTA).

Analytical Method: Quantitative

Specificité:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 3.8%, 3.5%, 2.0%
Inter-Assay Precision (CV%): 7.7%, 7.3%, 7.2%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Autre désignation: IL4R

Sujet:

Background: IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E (IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.

Gene Full Name: oncostatin M

ID gène: 791252

UniProt: Q6WG24

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process