IL-2 Kit ELISA

N° du produit ABIN8055987

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Cat IL-2.

Aperçu rapide pour IL-2 Kit ELISA (ABIN8055987)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Cat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156.0 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit IL-2 Kit ELISA

Seuil minimal de détection: 156.0 pg/mL

Fonction: Sandwich Feline IL-2 ELISA Kit to quantitate Feline IL-2 in cell culture supernatants, serum, plasma(heparin, EDTA).

Analytical Method: Quantitative

Specificité:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 20 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 3.8%, 2.7%, 7.7%
Inter-Assay Precision (CV%): 3.5%, 3.2%, 7.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2

Sujet:

Background: IL-2, Interleukin-2, formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin-or antigen-activated T cells. In situ hybridization, the IL-2 gene is assigned to 4q26-q28. IL2 can act as a growth hormone for both B and T lymphocytes. It is useful in the study of the molecular nature of T-cell differentiation and, like interferons, augments natural killer cell activity.

Gene Full Name: Interleukin-2

ID gène: 751114

UniProt: Q07885

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation