AGA Kit ELISA

N° du produit ABIN8068025

Kit ELISA AGA Humain, Colorimetric test pour la quantification de Humain AGA.

Aperçu rapide pour AGA Kit ELISA (ABIN8068025)

Antigène: AGA (Aspartylglucosaminidase (AGA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Gamme de detection: 1.37 units/mL - 1000 units/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit AGA Kit ELISA

Seuil minimal de détection: 1.37 units/mL

Fonction: The Aspartylglucosaminidase (AGA) Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG autoantibodies against AGA in human serum or plasma.

Analytical Method: Quantitative

Ingrédients:

Size / Description
• AGA Coated 96-Well Microplate
• 96 wells coated with AGA
• Wash Buffer
• Positive Control
• Negative Serum Control
• Biotinylated Anti-Human IgG
• HRP-Streptavidin
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent
• Sample Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Suggested Plasma Dilution: 500-fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control, negative control, or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated Anti-Human IgG Antibody into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: AGA (Aspartylglucosaminidase (AGA))

Autre désignation: Aspartylglucosaminidase

Sujet: Optional[Gene Symbols]: The Aspartylglucosaminidase (AGA) Human IgG ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with AGA protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgG antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-AGA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.