HDAC1 Kit ELISA

N° du produit ABIN8069029

Le kit ELISA HDAC1 (ABIN8069029) Humain et Souris est un kit ELISA Colorimetric conçu pour quantifier Humain et Souris HDAC1.

Aperçu rapide pour HDAC1 Kit ELISA (ABIN8069029)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Épitope: SUMOylated

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Lysate

Détail du produit HDAC1 Kit ELISA

Fonction: Sumoylated-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse Sumoylated-HDAC1 and HDAC1 in lysate samples.

Analytical Method: Quantitative

Ingrédients:

Size / Qty
• Anti-Pan-HDAC1 Microplate
• 96 wells
• Positive Control
• Anti-Sumoylation Detection Antibody
• Anti-HDAC1 Detection Antibody
• HRP-Streptavidin
• 200 μL
• Wash Buffer 1
• Assay Diluent B
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare positive control or sample dilutions
• Distilled or deionized water
• Shaker

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and Positive Control as instructed.
2. Add 100 μLsample and Positive Control to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC1

Sujet:

Gene Symbols: HDAC1, RPD3L1

Background: The Sumoylated-HDAC1 and Total-HDAC1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse sumoylated histone deacetylase 1 and total histone deacetylase 1. An anti-pan HDAC1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HDAC1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-sumoylation antibody is used to detect sumoylated HDAC1 or biotinylated anti-HDAC1 antibody is used to detect total HDAC1. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of sumoylated HDAC1 or total HDAC1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Histone deacetylase 1 (HD1) (EC 3.5.1.98) (Protein deacetylase HDAC1) (EC 3.5.1.-) (Protein decrotonylase HDAC1) (EC 3.5.1.-)

ID gène: 3065433759

UniProt: Q13547, O09106

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis