TRIM21 Kit ELISA

N° du produit ABIN8071167

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain TRIM21.

Aperçu rapide pour TRIM21 Kit ELISA (ABIN8071167)

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit TRIM21 Kit ELISA

Fonction: Sjogren Syndrome Antigen (SSA) Human IgM ELISA Kit is an in-vitro indirect ELISA for the quantitative measurement of Human IgM autoantibody against SSA in serum or plasma samples. This product is for research use only. Not for therapeutic or diagnostic applications.

Analytical Method: Quantitative

Ingrédients:

• Pre-coated 96-well microplate
• Wash Buffer
• Positive Control
• Biotinylated Detection Antibody
• Biotinylated Anti-Positive Control
• HRP-Streptavidin concentrate
• TMB One-Step Substrate
• Stop Solution
• Assay Diluent
• Sample Diluent

Matériel non inclus:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 μL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
4. Add 100 μL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Détail du antigène

Antigène: TRIM21 (Tripartite Motif Containing 21 (TRIM21))

Autre désignation: TRIM21

Sujet:

Gene Symbols: TRIM21 RNF81 RO52 SSA1

Protein Name (Synonyms): E3 ubiquitin-protein ligase TRIM21 (EC 2.3.2.27) (52 kDa Ro protein) (52 kDa ribonucleoprotein autoantigen Ro/SS-A) (RING finger protein 81) (Ro(SS-A)) (Sjoegren syndrome type A antigen) (SS-A) (Tripartite motif-containing protein 21)

ID gène: 6737

UniProt: Q5XPV5, Q96RF8