MIF Kit ELISA

N° du produit ABIN8083123

Kit ELISA MIF Humain, Colorimetric test pour la quantification de Humain MIF.

Aperçu rapide pour MIF Kit ELISA (ABIN8083123)

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit MIF Kit ELISA

Seuil minimal de détection: 0.312 ng/mL

Fonction: Human Macrophage Migration Inhibitory Factor (MIF) ELISA Kit for detecting MIF in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Sensibilité: 0.122 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Précision du teste: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C for one month, Long term: see manual.

Date de péremption: 12 months

Détail du antigène

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Autre désignation: Macrophage Migration Inhibitory Factor

Sujet: Gene Aliases: GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase

ID gène: 4282

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling