Angiotensin II Kit ELISA

N° du produit ABIN8089438

Kit ELISA Angiotensin II Boeuf (Vache), Colorimetric test pour la quantification de Boeuf (Vache) Angiotensin II.

Aperçu rapide pour Angiotensin II Kit ELISA (ABIN8089438)

Antigène: Angiotensin II (Ang II)

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 1.563 pg/mL - 100 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit Angiotensin II Kit ELISA

Seuil minimal de détection: 1.563 pg/mL

Fonction: Bovine Angiotensin II (Angiotensin II) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with ANGII , no obvious cross reaction with other analogues.

Sensibilité: 0.938 pg/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• HRP-Antigen(Concentrated
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Competitive ELISA, Coated with Antibody

Durée du test: 1 h

Plaque: Pre-coated

Protocole: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard/sample/HRP-labeled antigen working solution into each well. Seal the plate and statically incubate for 45 minutes at 37 °C. (Equilibrate the whole bottle of TMB for 30 min at 37 °C.) Washing: Wash the plate five times and immerse for 1 min each time. Step 3: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: Angiotensin II (Ang II)

Autre désignation: ANG II

Sujet: ANG II, Angiotensin II

UniProt: P01017

Pathways: Signalistation JAK/STAT, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity