GM-CSF Kit ELISA

N° du produit ABIN8126236

Le kit ELISA GM-CSF (ABIN8126236) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain GM-CSF.

Aperçu rapide pour GM-CSF Kit ELISA (ABIN8126236)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.9 pg/mL - 250 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit GM-CSF Kit ELISA

Seuil minimal de détection: 3.9 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 2.34 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human GMCSF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: < 2.34 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GMCSF (Granulocyte Macrophage Colony Stimulating Factor)

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin