IL1A Kit ELISA
Aperçu rapide pour IL1A Kit ELISA (ABIN8126246)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 15.6 pg/mL
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Fonction
- This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate.
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Analytical Method
- Quantitative
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-1a concentrations in serum, plasma. Detection Range: 15.6- 1000 pg/mL Sensitivity: < 9.4 pg / ml
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Plaque
- Pre-coated
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Protocole
- This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Stockage commentaire
- Please refer to the Manual
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- IL1A (Interleukin 1 alpha (IL1A))
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Autre désignation
- IL-1 Alpha (Interleukin-1 Alpha)
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Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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