IL-4 Kit ELISA

N° du produit ABIN8126250

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris IL-4.

Aperçu rapide pour IL-4 Kit ELISA (ABIN8126250)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-4 Kit ELISA

Seuil minimal de détection: 15.625 pg/mL

Fonction: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate.

Analytical Method: Quantitative

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma.  Detection Range: 15.625-1000pg/mL Sensitivity: <9.375pg/mL  

Information d'application

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: IL-4 (Interleukin 4)

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation