IL-5 Kit ELISA
Aperçu rapide pour IL-5 Kit ELISA (ABIN8126251)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 15.625 pg/mL
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Fonction
- This assay employs an antibody specific for Anti-mouse IL-5 coated on a 96-well plate.
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Analytical Method
- Quantitative
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-5 concentrations in serum, plasma. Detection Range: 15.625-1000pg/mL Sensitivity: <9.375pg/mL
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Plaque
- Pre-coated
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Protocole
- This assay employs an antibody specific for Anti-mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Stockage commentaire
- Please refer to the Manual
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- IL-5 (IL5) (Interleukin 5 (IL5))
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Autre désignation
- IL-5 (Interleukin 5)
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Pathways
- Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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