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CD13 Kit ELISA

ANPEP Reactivité: Humain Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN832246
  • Antigène Voir toutes CD13 (ANPEP) Kits ELISA
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Reactivité
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25-2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Fonction
    For the quantitative determination of human alanyl (membrane) aminopeptidase (ANPEP) concentrations in serum, plasma, tissue homogenates.
    Type d'échantillon
    Serum, Plasma, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of human ANPEP.
    Réactivité croisée (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensibilité
    7.81 pg/mL
    Ingrédients
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Indications d'application
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Commentaires

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Précision du teste
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Conseil sur la manipulation
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Date de péremption
    6 months
  • Antigène Voir toutes CD13 (ANPEP) Kits ELISA
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Abstract
    ANPEP Produits
    Synonymes
    APN Kit ELISA, CD13 Kit ELISA, GP150 Kit ELISA, LAP1 Kit ELISA, P150 Kit ELISA, PEPN Kit ELISA, A2AP Kit ELISA, AAP Kit ELISA, ALPHA-2-PI Kit ELISA, API Kit ELISA, PLI Kit ELISA, AAT1 Kit ELISA, ALT1 Kit ELISA, GPT1 Kit ELISA, Gpt1 Kit ELISA, Apn Kit ELISA, pepN Kit ELISA, SC8E4A.13 Kit ELISA, 1300007J06Rik Kit ELISA, 2310022B03Rik Kit ELISA, ALT Kit ELISA, Gpt-1 Kit ELISA, AP-M Kit ELISA, AP-N Kit ELISA, Cd13 Kit ELISA, Apm Kit ELISA, KZP Kit ELISA, Lap1 Kit ELISA, rAPN Kit ELISA, APDE Kit ELISA, p150 Kit ELISA, anpep Kit ELISA, fb64c05 Kit ELISA, wu:fb64c05 Kit ELISA, zgc:136771 Kit ELISA, alanyl aminopeptidase, membrane Kit ELISA, serpin family F member 2 Kit ELISA, glutamic--pyruvic transaminase Kit ELISA, aminopeptidase N Kit ELISA, aminopeptidase Kit ELISA, aminopeptidase n Kit ELISA, glutamic pyruvic transaminase, soluble Kit ELISA, alanyl (membrane) aminopeptidase Kit ELISA, membrane alanyl aminopeptidase Kit ELISA, alanyl (membrane) aminopeptidase b Kit ELISA, alanyl aminopeptidase, membrane L homeolog Kit ELISA, ANPEP Kit ELISA, SERPINF2 Kit ELISA, GPT Kit ELISA, Gpt Kit ELISA, Apn2 Kit ELISA, Apn1 Kit ELISA, SCO2643 Kit ELISA, pepN Kit ELISA, Shewana3_0897 Kit ELISA, Alvin_1024 Kit ELISA, Ndas_3413 Kit ELISA, Saut_1544 Kit ELISA, Fbal_1877 Kit ELISA, ML5_3424 Kit ELISA, Despr_1988 Kit ELISA, Mesop_5365 Kit ELISA, Anpep Kit ELISA, LOC101744250 Kit ELISA, anpepb Kit ELISA, anpep.L Kit ELISA
    Sujet
    Synonyms: APN, CD13, GP150, LAP1, P150, PEPN, aminopeptidase M|aminopeptidase N|membrane alanine aminopeptidase|microsomal aminopeptidase|myeloid plasma membrane glycoprotein CD13
    UniProt
    P15144
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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