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EPO Kit ELISA

EPO Reactivité: Souris AA 27-192 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate
N° du produit ABIN921054
  • Antigène Voir toutes EPO Kits ELISA
    EPO (Erythropoietin (EPO))
    Épitope
    AA 27-192
    Reactivité
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    46.9-3000 pg/mL
    Seuil minimal de détection
    46.9 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EPO
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: A27-R192
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <15pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: A27-R192
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

    Plaque
    Pre-coated
    Protocole
    mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
    • Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
    • Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
    • Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes EPO Kits ELISA
    EPO (Erythropoietin (EPO))
    Autre désignation
    EPO (EPO Produits)
    Synonymes
    EPO Kit ELISA, EP Kit ELISA, MVCD2 Kit ELISA, erythropoietin Kit ELISA, erythropoietin S homeolog Kit ELISA, erythropoietin a Kit ELISA, EPO Kit ELISA, epo Kit ELISA, epo.S Kit ELISA, Epo Kit ELISA, epoa Kit ELISA
    Classe de substances
    Hormone
    Sujet

    Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

    Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

    Synonyms: Erythropoietin,Epo,

    Full Gene Name: Erythropoietin

    Cellular Localisation: Secreted.
    ID gène
    13856
    UniProt
    P07321
    Pathways
    Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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