IL1A Kit ELISA

N° du produit ABIN921060

Ce kit ELISA Souris IL1A est un kit ELISA Colorimetric conçu pour quantifier Souris IL1A. Ce Kit ELISA a été cité dans 6+ publications.

Aperçu rapide pour IL1A Kit ELISA (ABIN921060)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Épitope: AA 115-270

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4.7-300 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit IL1A Kit ELISA

Seuil minimal de détection: 4.7 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-1 alpha

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli,S115-S270

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: S115-S270

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-1 alpha amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL mouse IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-1 alpha standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 37, Standard deviation: 1.924, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 89, Standard deviation: 3.83, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 187, Standard deviation: 7.3, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 46, Standard deviation: 2.99, CV(%): 6.5
• Sample 2: n=24, Mean(pg/ml): 98, Standard deviation: 5.3, CV(%): 5.4
• Sample 3: n=24, Mean(pg/ml): 175, Standard deviation: 10.85, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL1A Kit ELISA (ABIN921060)

Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." dans: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." dans: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." dans: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." dans: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).

Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." dans: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).

Kouadir, Yang, Tan, Shi, Lu, Zhang, Yin, Zhou, Zhao: "CD36 participates in PrP(106-126)-induced activation of microglia." dans: PLoS ONE, Vol. 7, Issue 1, pp. e30756, (2012) (PubMed).

Détail du antigène

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL1A

Sujet:

Protein Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18 kDa.

Synonyms: Interleukin-1 alpha,IL-1 alpha,Il1a,

Full Gene Name: Interleukin-1 alpha

Cellular Localisation: Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

ID gène: 16175

UniProt: P01582

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints